Repeats of additional cases from Fig. 2: (A) Pericytes were serum starved for 2 h and then treated with vehicle (lanes 1 and 7) or PDGF-BB (100 ng/mL) (lanes 2–6, and 8) for the indicated times and analysed by SDS-PAGE as in Fig. 2. Representative blots from two additional cases are shown. (B–F) Blots from (A) were analysed and quantified with Image Studio™. Phosphorylated PDGFRβ (Tyr751) (p-PDGFRβ) (B), PDGFRβ (C), and PDGF-BB (F) were normalized to GAPDH; phosphorylated Akt (Ser473) (p-Akt) (D) and phosphorylated ERK (Tyr204) (p-ERK) (E) were normalized to total Akt and ERK, respectively. (TIF 15997 kb)