ULBPs and Nec-2 ligand expression on SKO-007(J3) cells following treatment with JQ1. ULBPs and Nec-2 surface expression were analyzed by immunofluorescence and flow cytometry on SKO-007(J3) cells treated with JQ1 as described above for 72Â h. The grey-colored histograms represent basal expression of the indicated ligand, while thick black histograms represent the expression after treatment with the drug. Data are representative of three independent experiments. (TIF 3116Â kb)