Additional file 4: of Inhibition of bromodomain and extra-terminal (BET) proteins increases NKG2D ligand MICA expression and sensitivity to NK cell-mediated cytotoxicity in multiple myeloma cells: role of cMYC-IRF4-miR-125b interplay
Published in 2016 by
Maria Pia Abruzzese,
Maria Bilotta,
Cinzia Fionda,
Alessandra Zingoni,
Alessandra Soriani,
Elisabetta Vulpis,
Cristiana Borrelli,
Beatrice Zitti,
Maria Petrucci,
Maria Ricciardi,
Rosa Molfetta,
Rossella Paolini,
Angela Santoni,
Marco Cippitelli
Abstract
IRF4 and cMYC mRNA expression are inhibited in patient-derived MM PCs upon treatment with BETi. Real-time PCR analysis of total mRNA obtained from purified CD138+ cells untreated or treated with I-BET151 for 48 h in complete medium supplemented with 20 ng/ml IL-3 and 2 ng/ml IL-6. Data, expressed as fold change units, were normalized with GAPDH and referred to the untreated cells considered as calibrator. (TIF 4030 kb)