Knockdown of endogenous BRD1 using pools of small interfering RNAs (siRNAs). In order to downregulate the endogenous BRD1 level, (A) HEK293T cells were treated with two different pools of four different siRNA directed against BRD1 (BRD1 siRNA) and scrambled siRNA (Ctrl siRNA) as a control. Kits from Dharmacon (Method 1) and Qiagen (Method 2) were used at concentrations of 12.5 and 5.5 nM, respectively, and cells were harvested 24 h after transfection followed by QPCR measurement of BRD1 mRNA. BRD1 mRNA levels were significantly lower (approximately 70–80 % reduction for method 1 and 60 % for method 2) in cells treated with BRD1 directed siRNA compared to cells treated with scrambled siRNA (***P