Links

Tools

Export citation

Search in Google Scholar

Additional file 7 of Emergent material properties of developing epithelial tissues

This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Question mark in circle
Preprint: policy unknown
Question mark in circle
Postprint: policy unknown
Question mark in circle
Published version: policy unknown

Abstract

Figure S7. Live imaging and cell area and myosin measurements of ASGal4/UAS-ctMLCK embryos. A Confocal dorsal projections of amnioserosa tissue carrying membrane markers (DECadGFP, red) and myosin markers (zipperYFP, green) during slow dorsal closure of an example ASGal4/UAS-ctMLCK embryo. B Apical area and D myosin fluorescence intensity (f.i.) evolution for a sample cell in the dataset. The area trend and myosin minima trends are shown in orange. Resulting C apical strain and E rescaled (resc.) myosin for the raw signals (B) and (D), respectively. F Summary of stiffness and loss tangent of amnioserosa cells of wild-type and ASGal4/UAS-ctMLCK embryos. For wild-type embryos, the values of stiffness and loss tangent were broken down into four developmental stages as indicated in the figure. They are in arbitrary units (a.u.): stiffness wild type −1800–0 s: 1.90±0.21, loss tangent wild type −1800–0 s: 1.12±0.08, stiffness wild type 0–1800 s: 1.99±0.35, loss tangent wild type 0–1800 s: 1.11±0.1, stiffness wild type 1800–3600 s: 2.82±0.46, loss tangent wild type 1800–3600 s: 0.93±0.08, stiffness wild type 3600–4800 s: 3.51±0.43, loss tangent wild type 3600–4800 s: 0.85±0.05. Note that the stiffness and loss tangent shown for ASGal4/UAS-ctMLCK embryos (3.246±0.483 arbitrary units and 0.824±0.049 arbitrary units, respectively) correspond to the 0–1800-s developmental stage. Bars indicate 95 % confidence interval of experimental averages. (PDF 2877 kb)