Discrimination of specific w Pip alleles based on ank2 and pk1 markers. (A) three alleles: a (313 bp), b (217, 195, 98 bp) and c (293, 217 bp) after HinfI digestion of the ank2 PCR products; (B) three alleles: a/e (903, 430 bp), c (851, 498 bp) and d (497, 251, 107 bp) after TaqI digestion of the pk1 PCR products; (C) allele a (903, 303, 141 bp) after PstI digestion of the pk1 PCR products. (TIF 557 kb)