Influence of α and β on cleavage likelihood, neo-immunogenicity, and non-junction cleavage likelihood exemplified for spacers of length three. Cleavage likelihood and neo-immunogenicity decrease linearly with α. For the conservatively chosen α = 0.99, β influences neo-immunogenicity only marginally. Once α is further decreased, β influences neo-immunogenicity in a non-linear manner. Similar behavior can be seen for the non-junction cleavage likelihood. It decreases linearly with α and non-linearly with β. (PDF 51 kb)