Additional file 5: Figure S5. of MicroRNA-101 is repressed by EZH2 and its restoration inhibits tumorigenic features in embryonal rhabdomyosarcoma
Published in 2015 by
Serena Vella,
Silvia Pomella,
Pier Leoncini,
Marta Colletti,
Beatrice Conti,
Victor Marquez,
Antonio Strillacci,
Josep Roma,
Soledad Gallego,
Giuseppe Milano,
Maurizio Capogrossi,
Alice Bertaina,
Roberta Ciarapica,
Rossella Rota
Abstract
Signal-specific recruitment of EZH2 onto the miR-101-2 promoter in JR1 cells after EZH2 down-regulation. (A) ChIP assays on JR1 cells 72Â h after EZH2 or CTR siRNA transfection showing the recruitment of EZH2 and the levels of histone H3 trimethylation on Lys27 (H3K27me3) on miR-101-2, MCK, and SMAD6 (as negative control) regulatory regions. Normal rabbit IgG were used as negative control. Graphs represent the percent of immunoprecipitated material relative to input DNA. (B) mRNA levels (RT-qPCR) of pri-miR-101-2 in JR1 cells 48Â h after EZH2 siRNA treatment were normalized to GAPDH levels and expressed as fold increase over CTR siRNA.