Nur77 does not affect auto-ubiquitination of TRAF3 induced by LPS. Immunoprecipitation of endogenous TRAF3 from lysates of LPS-treated (50Â ng/ml) RAW264.7 cells expressing vector or myc-tagged Nur77 plasmid, immunoblotted for TRAF3 auto-ubiquitination with an anti-ubiquitin antibody. (PDF 62Â kb)