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Oxford University Press, Nucleic Acids Research, 14(30), p. 3235-3244

DOI: 10.1093/nar/gkf441

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Analysis of repeatability in spotted cDNA microarrays

Journal article published in 2002 by Mette Langaas, Winston P. Kuo, Birgitte Smith-Sørensen, Ola Myklebost, Tor-Kristian Jenssen, Eivind Hovig ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We report a strategy for analysis of data quality in cDNA microarrays based on the repeatability of repeatedly spotted clones. We describe how repeatability can be used to control data quality by developing adaptive filtering criteria for microarray data containing clones spotted in multiple spots. We have applied the method on five publicly available cDNA microarray data sets and one previously unpublished data set from our own laboratory. The results demonstrate the feasibility of the approach as a foundation for data filtering, and indicate a high degree of variation in data quality, both across the data sets and between arrays within data sets.