Soccer matches are normally played over 90 minutes, however it is common for players to encounter a period of extra-time (ET; 30 min) during knockout competitions such as the FIFA World Cup. Despite the importance of ET in terms of deciding the match outcome, there is a paucity of data investigating responses to ET. Notably, no investigation has attempted to profile the neuromuscular fatigue response. Thus, the precise mechanisms of neuromuscular fatigue during prolonged soccer-specific exercise are unknown.