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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, Acta Cirurgica Brasileira, 9(31), p. 629-637

DOI: 10.1590/s0102-865020160090000009

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Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model

Journal article published in 2016 by Tirapelli,Daniela Pretti da Cunha, Daniela Pretti da Cunha Tirapelli, Rodrigues,Andressa Romualdo, Andressa Romualdo Rodrigues, Lizarte Neto,Fermino Sanches, Camila Albuquerque Melo de Carvalho, Silva,Jairo Pinheiro, Paulo Cézar Novais ORCID, Colli,Benedicto Oscar, Jairo Pinheiro Silva, Fermino Sanches Lizarte Neto, Carvalho,Camila Albuquerque Melo De, Novais,Paulo Cézar, Carlos Gilberto Carlotti Júnior, Luís Fernando Tirapelli and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT PURPOSE: To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism. METHODS: Fifty Wistar rats were used and divided into: control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study. RESULTS: Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA. CONCLUSION: We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.