ABSTRACT The objective of this study was to evaluate the growth of rubber clones GT1, PR255, RRIM600 grown on different substrates and environments for the production of rubber rootstocks. The experiment was conducted at the Universidade Estadual de Mato Grosso do Sul, Cassilândia University Unit, in Cassilândia-MS. The treatments were conducted in 3 environments: (A1): Sombrite®; (A2) Aluminet® and (A3) full sun, where the clones being tested were: GT1; PR 255 and RRIM 600, grown in substrates: pure soil; soil + cattle manure and commercial substrate usually used for forestry. The experimental design was a randomized block in a factorial 3 x 3 (three substrates x three clones), with 4 replicates with 10 seedlings of rubber trees per plot. The use of commercial substrate and soil + cattle manure in the ratio (7:1) favored the development of rubber rootstock seedlings with taller plants, more leave number, higher mass and higher quality index. For the formation of rootstocks, the variety RRIM600 was the one that showed better agronomic characteristics in the formation of rubber tree seedlings, with higher number of leaves, tallest plants, larger mass and higher quality index. The use of Sombrite® and Aluminet® screens contributed positively to the formation of rootstocks with larger root and above-ground masses. The plants produced in the Aluminet® screen showed higher number of leaves.