ABSTRACT Intensive land use can affect macrofaunal biodiversity, which is a property that can be used as a soil quality indicator. This study evaluated the abundance and diversity of soil macrofauna and its relation to soil chemical and physical properties in five land use systems (LUS) in the eastern region of Santa Catarina. The following LUS were studied: native forest (NF), eucalyptus plantations (EP), perennial pasture (PP), integrated crop-livestock (ICL), and no-tillage cropping (NT). The macrofauna was quantified in 0.25 × 0.25 m monoliths and sampled in the 0.00-0.20 m layer in the summer (Jan/2012) and winter (Jul/2012). For each LUS, nine points were sampled, distributed in a 30 × 30 m sampling grid. After screening the edaphic macrofauna organisms, the individuals were counted and identified at the species level when possible, or in major taxonomic groups. The Shannon diversity indices were calculated and the macrofaunal groups together with the physical and chemical properties were subjected to principal component (PCA) and redundancy analysis (RDA). The abundance and diversity of macrofaunal groups are affected by the LUS. The properties of organic matter, macroporosity, bulk density, cation exchange capacity at pH 7.0, base saturation, potential acidity, and exchangeable Al were related to the abundance of soil macrofaunal groups. The stability and biodiversity of soil macrofauna were highest in the LUS of NF, PP, and EP.