Dissemin is shutting down on January 1st, 2025

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Nature Research, Nature Methods, 10(3), p. 793-796, 2006

DOI: 10.1038/nmeth929

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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

Journal article published in 2006 by Michael J. Rust, Mark Bates ORCID, Xiaowei Zhuang
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.