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American Society for Microbiology, Journal of Virology, 17(87), p. 9928-9932, 2013

DOI: 10.1128/jvi.01259-13

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Expression Strategy of Aedes albopictus Densovirus

Journal article published in 2013 by Hanh T. Pham, Françoise-Xavière Jousset, Jonathan Perreault ORCID, Hiroko Shike, Jozsef Szelei, Max Bergoin, Peter Tijssen
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.