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QuaNCAT: quantitating proteome dynamics in primary cells.
Journal article published in 2013 by
Aj Howden,
Vincent Geoghegan,
Kristin Katsch,
Georgios Efstathiou 
,
Bhaskar Bhushan ,
Omar Boutureira,
Benjamin Thomas,
Dc Trudgian,
Bm Kessler,
Dc Dieterich,
Bg Davis,
Oreste Acuto
,
Bhaskar Bhushan ,
Omar Boutureira,
Benjamin Thomas,
Dc Trudgian,
Bm Kessler,
Dc Dieterich,
Bg Davis,
Oreste Acuto
Abstract
Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli. © 2013 Nature America, Inc. All rights reserved.