Published in

Nature Research, Scientific Reports, 1(6), 2016

DOI: 10.1038/srep24484

Links

Tools

Export citation

Search in Google Scholar

Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Red circle
Postprint: archiving forbidden
Green circle
Published version: archiving allowed
Data provided by SHERPA/RoMEO

Abstract

結晶を損傷しない新しいタンパク質結晶の輸送媒体を発見-タンパク質の結晶構造解析で新薬創生に一歩近づく. 京都大学プレスリリース. 2016-04-22. ; The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.