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Oxford University Press, Nucleic Acids Research, 22(42), p. 13525-13533, 2014

DOI: 10.1093/nar/gku1147

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An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

Journal article published in 2014 by Thomas Bock ORCID, Wei-Hua Chen, Alessandro Ori ORCID, Nayab Malik, Noella Silva-Martin, Jaime Huerta-Cepas, Sean T. Powell, Panagiotis L. Kastritis ORCID, Georgy Smyshlyaev, Ivana Vonkova, Joanna Kirkpatrick, Tobias Doerks, Leo Nesme, Jochen Baßler, Martin Kos and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.