Published in
Oxford University Press, The Plant Cell, 4(24), p. 1316-1326, 2012
Links
- Oxford University Press
- ORCID
- ORCID
- [www.ncbi.nlm.nih.gov] | PDF
- [radar.brookes.ac.uk]
- [wrap.warwick.ac.uk]
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
Tools
Secretory Pathway Research: The More Experimental Systems the Better
,
Lorenzo Frigerio ,
Chris Hawes,
Inhwan Hwang,
Jaideep Mathur,
Jean-Marc Neuhaus,
David G. Robinson
Full text: Download
Abstract
Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.