Accumulation and distribution of two types of phenolic antifeedant metabolites, vismiones and ferruginins, in specialized cells were investigated in Vismia guianensis DC. Chemical, light, and electron-microscope analyses revealed that the two types of compounds were produced in different cells and organs and at different stages of plant development. Vismiones accumulated at various concentrations in the leaf, depending on its developmental stage, as well as in the stem of very young plants. Ferruginins appeared late in the plant's development and accumulated exclusively in secretory ducts in the secondary body of the stem and in secretory cavities of the fruits.