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Hindawi, Journal of Biomedicine and Biotechnology, (2009), p. 1-6, 2009

DOI: 10.1155/2009/179106

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Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

Journal article published in 2009 by Renata Angeli, Maria Tereza dos Santos Correia, Nathalia V. N. da Paz, Jackeline C. Maciel, Flávia F. B. Araújo, Patrícia M. G. Paiva ORCID, Glícia M. T. Calazans, Ana Paula Valente, Fábio C. L. Almeida ORCID, Luana C. B. B. Coelho, Luiz B. Carvalho, Maria da Paz C. Silva, Maria Tereza dos Santos Correia
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.