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American Society for Microbiology, Applied and Environmental Microbiology, 10(75), p. 3362-3365, 2009

DOI: 10.1128/aem.00336-09

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Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

ABSTRACT Novel arsenite [As(III)] oxidase structural genes ( aoxAB ) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.