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American Society for Microbiology, Journal of Clinical Microbiology, 4(50), p. 1437-1439, 2012

DOI: 10.1128/jcm.06746-11

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Comparative Study Using Phenotypic, Genotypic, and Proteomics Methods for Identification of Coagulase-Negative Staphylococci

Journal article published in 2012 by Anne J. M. Loonen, Arjan R. Jansz, Jeandery N. B. Bergland, Marion Valkenburg, Petra F. G. Wolffs ORCID, Adriaan J. C. van den Brule
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Five methods were compared to determine the most accurate method for identification of coagulase-negative staphylococci (CoNS) ( n = 142 strains). Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) showed the best results for rapid and accurate CoNS differentiation (99.3% of strains correctly identified). An alternative to this approach could be Vitek2 combined with partial tuf gene sequencing (100% of strains correctly identified when both methods are performed simultaneously).