Electrochemistry has been introduced as a powerful tool in order to prepare new organometallic reagents for functionalizing of mesoporous silica. Preparation of the reagents was based on electrochemical oxidation of dihydroxybenzene derivatives in the presence of 3-(trimethoxysilyl)-1-propanethiol as a nucleophile. The mechanisms of electrochemical reactions were studied by voltammetric studies. Mesoporous silica SBA-15 was also synthesized in this work through sol-gel hydrothermal method using Genapol PF-10 as structure directing compound. The prepared mesoporous silica was characterized by FT-IR analysis and Barrett–Joiner–Halenda (BJH) pore size and Brunauer–Emmett–Teller (BET) surface area measurement methods using N₂adsorption–desorption isotherm. Finally, the organometallic reagent was covalently grafted on the surface of mesoporous silica. Functionalizing of this material with the new reagent was confirmed by Fourier transform infrared (FT-IR) spectroscopy. The functionalized mesoporous silica by new reagent can be utilized in biological applications.