Generally in the anterior cruciate ligament (ACL) injury assessment specific laxity tests (i.e., Lachman, drawer tests) are clinically performed to evaluate the presence of ligamentous lesion. At present these tests are qualitatively evaluated by the surgeon and some quantitative measurements can be performed only for Lachman/drawer tests by means of dedicated devices. This study aimed to characterize the accuracy of a novel integrated optical-based method that can be used both in intra-operative and in office assessment of ACL injuries; in particular this technology was addressed to measure the loads/torques applied during clinical laxity tests, extending the current possible quantitative evaluations. The system, based on a commercial optical localizer and common springs, was spatially characterized in order to verify displacement/rotation and corresponding applied load/torque measurements. Evaluated limits of agreement between measured and applied loads were from –0.541 to 1.781 N, with a bias of 0.621 N (P = 0.05) in a dedicated clinical-like setup. This approach reported an excellent accuracy in load measurements, showing its possible integration in computer-aided surgery (CAS).