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American Chemical Society, Journal of the American Chemical Society, 38(133), p. 14936-14939, 2011

DOI: 10.1021/ja206626g

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Site-specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome

Journal article published in 2011 by Ishu Saraogi, Dawei Zhang, Sandhya Chandrasekaran ORCID, Shu-Ou Shan
This paper is available in a repository.
This paper is available in a repository.

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Abstract

As newly synthesized proteins emerge from the ribosome, they interact with a variety of cotranslational cellular machineries that facilitate their proper folding, maturation, and localization. These interactions are essential for proper function of the cell, and the ability to study these events is crucial to understanding cellular protein biogenesis. To this end, we have developed a highly efficient method to generate ribosome-nascent chain complexes (RNCs) site-specifically labeled with a fluorescent dye on the nascent polypeptide. The fluorescent RNC provides real-time, quantitative information on its cotranslational interaction with the signal recognition particle and will be a valuable tool in elucidating the role of the translating ribosome in numerous biochemical pathways.