Published in
Centers for Disease Control and Prevention, Emerging Infectious Diseases, 1(13), p. 73-81, 2007
Links
- ORCID
- ORCID
- Centers for Disease Control and Prevention
- [edoc.rki.de] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [citeseerx.ist.psu.edu] | PDF
- [www.cdc.gov] | PDF
- [europepmc.org] | PDF
- [www.nbn-resolving.de] | PDF
Tools
Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases
,
Phenix-Lan Quan,
Omar J. Jabado,
Sean Conlan,
David L. Hirschberg,
Yang Liu,
Junhui Zhai,
Neil Renwick,
Jeffrey Hui,
Hedi Hegyi,
Allen Grolla,
James E. Strong,
Jonathan S. Towner,
Thomas W. Geisbert,
Peter B. Jahrling
and other authors.
Full text: Download
Abstract
To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.