Convective boiling in narrow channels may under specific conditions display an unsteady behavior. An experimental set-up has been elaborated to investigate heat and mass transfer and to analyze two-phase flow instabilities in rectangular microchannels with a hydraulic diameter of 889 μm. Depending on the operating conditions two types of behavior are observed: a steady state characterized by pressure drop fluctuations with low amplitudes (from 0.5 to 5 kPa/m) and no characteristic frequency; a non-stationary state of two-phase flow. The pressure signals exhibit fluctuations with high amplitudes (from 20 to 100 kPa/m) and frequencies ranging from 3.6 to 6.6 Hz. Steady and unsteady thermo-hydraulic behaviors depending on the two control parameters (heat flux and mass velocity) are analyzed and given in this paper.