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Cold Spring Harbor Protocols, 8(2013), p. pdb.prot073031-pdb.prot073031

DOI: 10.1101/pdb.prot073031

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Live Cell Imaging of Cytoplasmic and Nuclear Ca2+ Dynamics in Arabidopsis Roots

Journal article published in 2013 by Melanie Krebs ORCID, Karin Schumacher
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Approaches for Ca2+ measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca2+ dynamics in Arabidopsis lines expressing Yellow Cameleon Ca2+ indicators.