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Elsevier, Journal of Biological Chemistry, 22(289), p. 15426-15440, 2014

DOI: 10.1074/jbc.m113.539148

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Neurolysin Knockout Mice Generation and Initial Phenotype Characterization*

Journal article published in 2014 by Diogo M. L. P. Cavalcanti ORCID, Leandro M. Castro, José C. Rosa Neto, Marilia Seelaender, Rodrigo X. Neves, Vitor Oliveira, Fábio L. Forti, Leo K. Iwai, Fabio C. Gozzo, Mihail Todiras, Ines Schadock ORCID, Carlos C. Barros, Michael Bader, Emer S. Ferro
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The oligopeptidase neurolysin (EC 3.4.24.16; Nln) was first identified in rat brain synaptic membranes and shown to ubiquitously participate in the catabolism of bioactive peptides such as neurotensin and bradykinin. Recently, it was suggested that Nln reduction could improve insulin sensitivity. Here, we have shown that Nln knockout mice (KO) have increased glucose tolerance, insulin sensitivity and gluconeogenesis. KO mice have increased liver mRNA for several genes related to gluconeogenesis. Isotopic label semi-quantitative peptidomic analysis suggests increase in specific intracellular peptides in gastrocnemius and epididymal adipose tissue, which likely is involved with the increased glucose tolerance and insulin sensitivity in the KO mice. These results suggest the exciting new possibility that Nln is a key enzyme for energy metabolism and could be a novel therapeutic target to improve glucose uptake and insulin sensitivity.