Published in
BioMed Central, Arthritis Research and Therapy, 1(12), p. R9
DOI: 10.1186/ar2908
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Chronic arthritis leads to disturbances in the bone collagen network
,
Joana Duarte,
Rita Cascão ,
Ana Rodrigues,
Inês P. Perpétuo,
Saba Abdulghani ,
Pedro M. Amaral,
Shimon Sakaguchi,
Yrjö T. Konttinen,
Luís Graça ,
Maria F. Vaz,
João E. Fonseca
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Abstract
Abstract Introduction In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by chronic inflammation is associated with an imbalance in bone turnover and also a disorganization of the bone type I collagen network. Methods Serum, vertebrae and femur bones were collected from eight-month-old polyarthritis SKG mice and controls. Strength of the femoral bones was evaluated using three-point bending tests and density was assessed with a pycnometer. Bone turnover markers carboxy-terminal collagen cross-linking telopeptides (CTX-I) and amino-terminal propeptide of type I procollagen (PINP) were measured in serum. The organization and density of bone collagen were analyzed in vertebrae using second-harmonic generation (SHG) imaging with a two-photon microscope and trabecular bone microstructure was assessed by scanning electron microscope (SEM). Results Femoral bones of SKG mice revealed increased fragility expressed by deterioration of mechanical properties, namely altered stiffness ( P = 0.007) and reduced strength ( P = 0.006), when compared to controls. Accordingly, inter-trabecular distance and trabecular thickness as observed by SEM were reduced in SKG mice. PINP was significantly higher in arthritic mice (9.18 ± 3.21 ng/ml) when compared to controls (1.71 ± 0.53 ng/ml, P