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National Academy of Sciences, Proceedings of the National Academy of Sciences, 19(84), p. 6810-6814, 1987

DOI: 10.1073/pnas.84.19.6810

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Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing.

Journal article published in 1987 by B. Seraphin, B. Séraphin ORCID, A. Boulet, M. Simon, G. Faye
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We have constructed a respiring yeast strain devoid of mitochondrial introns to screen nuclear pet- mutants for those that play a direct role in mitochondrial intron excision. Intron-less mitochondria are introduced by cytoduction into pet- strains that have been made rho0; cytoductants therefrom recover respiratory competency if the original pet- mutation is required only for mitochondrial splicing. By this means, we have identified 11 complementation groups of such genes. Their total number may be estimated as about 18.