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Springer (part of Springer Nature), Bulletin of Environmental Contamination and Toxicology, 3(86), p. 278-282

DOI: 10.1007/s00128-011-0205-3

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Fast cleanup system combined with a dioxin-responsive element-driven luciferase bioassay for analysis of polychlorinated dibenzo-p-dioxins/furans in sediments and soils

Journal article published in 2011 by How-Ran Chao, Ya-Fen Wang, Ding-Yan Lin, Yi-Ting Cheng, Tsui-Chun Tsou ORCID, HR;Wang YF;Lin DY;Cheng YT;Tsou TC Chao
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A green technique was designed for assessing toxic equivalence (TEQ) levels of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in sediments and soil from dioxin-contaminated areas. This technique combines a fast cleanup system with a dioxin-responsive-element (DRE)-driven luciferase bioassay. Samples from sediment (n = 10) and soil (n = 11) were analyzed with the technique; levels of PCDD/Fs ranged from 75.1 to 2,670 ng DRE-driven luciferase activity (DL)-TEQ/kg dry weight (d.w.). Significant correlations were found between DL-TEQs (by the bioassay) and PCDD/F WHO-TEQs (by HRGC/HRMS). DL-TEQs is significantly correlated with WHO-TEQs of 2,3,7,8-TCDD and 1,2,3,7,8-PeCDD using a multiple stepwise linear regression model (adjusted R(2) = 0.962, p < 0.001).