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Public Library of Science, PLoS ONE, 3(6), p. e17767, 2011

DOI: 10.1371/journal.pone.0017767

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Phytophthora infestans Has a Plethora of Phospholipase D Enzymes Including a Subclass That Has Extracellular Activity

Journal article published in 2011 by Harold J. G. Meijer ORCID, Hussen Harrun Hassen, Francine Govers ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol.