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American Society for Microbiology, Journal of Virology, 10(85), p. 5220-5223, 2011

DOI: 10.1128/jvi.00284-11

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Clonal Sequences Recovered from Plasma from Patients with Residual HIV-1 Viremia and on Intensified Antiretroviral Therapy Are Identical to Replicating Viral RNAs Recovered from Circulating Resting CD4+ T Cells

Journal article published in 2011 by Jeffrey A. Anderson, Nancie M. Archin, William Ince, Daniel Parker, Ann Wiegand, John M. Coffin, JoAnn Kuruc, Joseph Eron, Ronald Swanstrom, David M. Margolis ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4 + T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV plasma was performed, and the sequences were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4 + T cells. The circulating LLV virus clone was identical to virus recovered from outgrowth assays from pools of millions of resting CD4 + T cells. Understanding the sources of LLV requires evaluation of all possible reservoirs of persistent HIV infection.