American Society for Microbiology, Applied and Environmental Microbiology, 7(71), p. 4117-4120, 2005
DOI: 10.1128/aem.71.7.4117-4120.2005
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ABSTRACT Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.