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适用于PCR的棉花基因组DNA快速、批量制备方法的探讨/Fast and Batch Process of Cotton Genomic DNA Preparation Methods for PCR Analysis[J]

This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

通过筛选和优化传统CTAB SDS法的操作步骤,建立了一种快速、批量提取棉花基因组DNA的方法.结果表明:利用该方法提取的DNA含量高,能够满足PCR的要求.初步解决了棉花基因组DNA提取步骤复杂、耗时耗力的问题,为转基因作物育种领域的分子检测提供可行的方法.