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Oxford University Press, Nucleic Acids Research, 12(30), p. 2609-2619, 2002

DOI: 10.1093/nar/gkf374

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Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines

Journal article published in 2002 by Beatriz García de la Torre, A. Avi{\~n}{ó}, Miriam Frieden, Juan Carlos Morales, B. G. De La Torre, Ramón Güimil García, Ramón Güimil García, Ferran Azorín, José Luis Gelpí ORCID, Modesto Orozco, Carlos González, Ramon Eritja ORCID, Anna Aviñó, A. Avino
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.