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Cold Spring Harbor Laboratory Press, RNA, 9(13), p. 1516-1527, 2007

DOI: 10.1261/rna.609807

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Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation

Journal article published in 2007 by Christophe Dez, Mensur Dlakić ORCID, David Tollervey ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A family of HEAT-repeat containing ribosome synthesis factors was previously identified in Saccharomyces cerevisiae. We report the detailed characterization of two of these factors, Utp10 and Utp20, which were initially identified as components of the small subunit processome. Coprecipitation analyses confirmed the association of Utp10 and Utp20 with U3 snoRNA and the early pre-rRNA processing intermediates. Particularly strong association was seen with aberrant processing intermediates, which may help target these RNAs for degradation. Genetic depletion of either protein inhibited the early pre-rRNA processing steps in 18S rRNA maturation but had little effect on pre-rRNA transcription or synthesis of the 25S or 5.8S rRNAs. The absence of the poly(A) polymerase Trf5, a component of the TRAMP5 complex and exosome cofactor, led to stabilization of the aberrant 23S RNA in strains depleted of Utp10 or Utp20. In the case of Utp10, 20S pre-rRNA synthesis was also modestly increased by this loss of surveillance activity.