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Investigations in Yeast Functional Genomics and Molecular Biology, p. 233-260

DOI: 10.1201/b16586-13

Public Library of Science, PLoS ONE, 11(5), p. e15499, 2010

DOI: 10.1371/journal.pone.0015499

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A Screen for RNA-Binding Proteins in Yeast Indicates Dual Functions for Many Enzymes

Journal article published in 2010 by Tanja Scherrer, Nitish Mittal, Sarath Chandra Janga ORCID, André P. Gerber
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.