Thalassotalea sp. strain ND16A belongs to the family Colwelliaceae and was isolated from eastern Mediterranean Sea water at a depth of 1,055 m. Members of Colwelliaceae are ubiquitous marine heterotrophs. Here, we report the draft genome sequence of Thalassotalea sp. strain ND16A, a member of the newly described genus Thalassotalea. FOOTNOTES Address correspondence to Stephen M. Techtmann, stephen.techtmann{at}gmail.com, or Terry C. Hazen, tchazen{at}utk.edu. Citation Stelling SC, Techtmann SM, Utturkar SM, Alshibli NK, Brown SD, Hazen TC. 2014. Draft genome sequence of Thalassotalea sp. strain ND16A isolated from eastern Mediterranean Sea water collected from a depth of 1,055 meters. Genome Announc. 2(6):e01231-14. doi:10.1128/genomeA.01231-14. Received 16 October 2014. Accepted 21 October 2014. Published 26 November 2014. Copyright © 2014 Stelling et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.