Published in
Wiley, Biopolymers, 3(99), p. 196-202, 2012
DOI: 10.1002/bip.22106
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- [europepmc.org] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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Sweet entanglements-protein: Glycan interactions in two HIV-inactivating lectin families
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Abstract
Structures and sugar binding by members of two lectin families, CVNH and OAAH, were determined to elucidate the basis for recognition of high-mannose glycans on the HIV envelope glycoprotein gp120. We solved NMR solution and/or crystal structures for several lectins and delineated their carbohydrate specificity by array screening and direct NMR titrations. Both families recognize different epitopes on high-mannose glycans, namely Manα(1–2)Man units at the ends of the D1 and D3 arms and α3,α6-mannopentaose at the central branch point of Man-8 or Man-9 for CVNH and OAAH lectins, respectively.