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American Society for Microbiology, Journal of Clinical Microbiology, 2(46), p. 792-795, 2008

DOI: 10.1128/jcm.01470-07

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Dual-Reporter Phenotypic Assay for Human Immunodeficiency Viruses

Journal article published in 2007 by K. Kajiwara, E. Kodama, Y. Sakagami, T. Naito, M. Matsuoka ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

ABSTRACT We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled β-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.