Published in
Elsevier, Journal of Microbiological Methods, (103), p. 25-28, 2014
DOI: 10.1016/j.mimet.2014.04.014
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Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae
,
Manuela de Pablos,
José Luis García,
Ernesto García,
Pedro García,
José Manuel Pingarrón ,
Jesús Mingorance ,
Susa Campuzano
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Abstract
A disposable PCR-based amperometric magneto-genosensor for detection and identification of Streptococcus pneumoniae was evaluated. ROC curve analysis used to determine optimal signal cutoff values yielded a sensitivity of 91 % and a specificity of 90 %. The method was also tested for the direct detection of pneumococci in clinical samples. ; 15 p.-3 fig.-2 tab. ; This work was funded by a grant from the Ministerio de Ciencia e Innovación (IPT-2011-1337-010000). CIBER de Enfermedades Respiratorias (CIBERES) is an initiative of the Instituto de Salud Carlos III. The financial support of the AVANSENS Program from the Comunidad de Madrid (S2009PPQ-1642) is gratefully acknowledged.