This paper is devoted to production of metallic micro-and nanowires (Cu, Co, Ni and Fe) using commercial track membranes. Specially prepared matrices were also fabricated and used for this purpose. The process of electrodeposition of these metals into the nanosized pores was investigated and found to be non-linear for small pores. The obtained ensembles of nanowires could be used as the effective templates for emission of molecules in mass-spectrometry. Mass-spectra of test peptide (gramicidin deposited on substrate - ensemble of copper nanowires) was obtained and investigated in different conditions.