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International Union of Crystallography, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 8(68), p. 923-926, 2012

DOI: 10.1107/s1744309112025651

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Cloning, expression, purification and preliminary X-­ray analysis of a putative metagenome-derived lipase

Journal article published in 2012 by Francesco Fersini, Yuliya Dall’Antonia, Jennifer Chow, Wolfgang R. Streit ORCID, Jochen Mueller-Dieckmann
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A putative lipase, LipS, from the soil metagenome of an environmental sample taken at the Biocenter Klein Flottbek, Hamburg, Germany was cloned, expressed and purified. Two different constructs of the protein were crystallized in a search for well diffracting crystals. Preliminary X-ray analyses indicated that crystals in space groups P42212 and P4 diffracted X-ray radiation to 2.8 and 2.0 Å resolution, respectively.