Here we report the development of Poly Urethane (PU)/ graphene oxide composite used as the scaffold of anterior cruciate ligament tissue engineering. The influences of the GO on the composite were studied by FTIR, SEM and XRD. The Youngs modulus of the composite is 30.6 MPa compared to that of PU`s 6.8 MPa. The GO induced heterogeneous crystal increasing should be the reason for the composite modulus improving, as well as the interaction between the GO and PU. The good biocompatibility of the composite testified by cell viability experiment indicates the promising candidates of the material as the ACL tissue engineering scaffold.