A modified electrospinning setup with two-grounded-frame collector is proposed to fabricate aligned fiber arrays and fiber ropes. In this setup, two frames are placed under the spinneret, with the outer frame rotated with an electromotor and the inner frame hold still in a horizontal direction. Aligned nanofiber arrays can be collected rapidly on the inner frame. Influence of included angle and motor rotating speed on the arrays is discussed. In addition, through rotating one side of the inner frame, twisted fiber ropes with diameter 30~40 μm and length of 12 cm are obtained. Mechanical properties of the individual nanofiber ropes are also measured and discussed.