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American Society for Microbiology, Journal of Clinical Microbiology, 9(47), p. 2985-2988, 2009

DOI: 10.1128/jcm.00850-09

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Rapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray

Journal article published in 2009 by Astrid Gall ORCID, Bernd Hoffmann, Timm Harder, Christian Grund, Ralf Ehricht, Martin Beer
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.